Many people applying for academic jobs this season are well into the process. Interviews are happening across the country and more are on the way. If you're putting together a talk for an on campus interview, you'll want to make sure you're communicating to your audience the information they'll need to make a decision. Obviously, it's not all going to come down to your talk, but many faculty will only see you during this time.
The most critical piece, IMO, is figuring out whether you need to include a full Future Directions section in your feature talk. I've seen it done several ways, but what you present on the work you plan to do at the hiring institution is obviously the key. In my current job, the future directions was a separate talk. I still gave a 5 minute compressed version for those who wouldn't see the second talk, but it was just a teaser.
If you're asked to put everything in one talk, I would roughly break it down like:
30 min of highlighting what you have done
20 min of talking about what you will do
10 min for questions
People often say that their completed work is either too much or too diverse to cover in 30 or 40 or 50 minutes. This is good and probably why you are being interviewed. However, it is up to YOU to create a narrative about who you are and what you bring to the table. What led you from one training environment to another*? What is the flow to your science and what you have accomplished? What are you going to take from that and apply to your new awesome lab?
Your audience should come away with a feel for the flow and trajectory of your career. That is your take home for them. You want them to think "Wow, she did all that and is ready to really take off on an original path!"
Look at the structure of your talk and make sure that is front and foremost.
* Or, what is the best possible spin you can apply post hoc?